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Sample preparation and laser irradiation of the pupal terminalia. The puparium at the abdominal tip was removed to expose the developing genitalia. The pupa was placed onto <t>a</t> <t>glass-bottom</t> petri dish through an angled hole made in a drop of solidified agarose. An infrared laser (1,480 nm) was applied through the objective lens and irradiated the target cells at a certain distance (depth) from the glass.
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Sample preparation and laser irradiation of the pupal terminalia. The puparium at the abdominal tip was removed to expose the developing genitalia. The pupa was placed onto <t>a</t> <t>glass-bottom</t> petri dish through an angled hole made in a drop of solidified agarose. An infrared laser (1,480 nm) was applied through the objective lens and irradiated the target cells at a certain distance (depth) from the glass.
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Sample preparation and laser irradiation of the pupal terminalia. The puparium at the abdominal tip was removed to expose the developing genitalia. The pupa was placed onto <t>a</t> <t>glass-bottom</t> petri dish through an angled hole made in a drop of solidified agarose. An infrared laser (1,480 nm) was applied through the objective lens and irradiated the target cells at a certain distance (depth) from the glass.
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Sample preparation and laser irradiation of the pupal terminalia. The puparium at the abdominal tip was removed to expose the developing genitalia. The pupa was placed onto <t>a</t> <t>glass-bottom</t> petri dish through an angled hole made in a drop of solidified agarose. An infrared laser (1,480 nm) was applied through the objective lens and irradiated the target cells at a certain distance (depth) from the glass.
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glass bottom dishes  (World Precision Instruments)
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Sample preparation and laser irradiation of the pupal terminalia. The puparium at the abdominal tip was removed to expose the developing genitalia. The pupa was placed onto <t>a</t> <t>glass-bottom</t> petri dish through an angled hole made in a drop of solidified agarose. An infrared laser (1,480 nm) was applied through the objective lens and irradiated the target cells at a certain distance (depth) from the glass.
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Sample preparation and laser irradiation of the pupal terminalia. The puparium at the abdominal tip was removed to expose the developing genitalia. The pupa was placed onto a glass-bottom petri dish through an angled hole made in a drop of solidified agarose. An infrared laser (1,480 nm) was applied through the objective lens and irradiated the target cells at a certain distance (depth) from the glass.

Journal: G3: Genes | Genomes | Genetics

Article Title: Heat-mediated manipulation of gene expression by IR-LEGO in the developing genitalia in Drosophila

doi: 10.1093/g3journal/jkag035

Figure Lengend Snippet: Sample preparation and laser irradiation of the pupal terminalia. The puparium at the abdominal tip was removed to expose the developing genitalia. The pupa was placed onto a glass-bottom petri dish through an angled hole made in a drop of solidified agarose. An infrared laser (1,480 nm) was applied through the objective lens and irradiated the target cells at a certain distance (depth) from the glass.

Article Snippet: The puparium at the abdominal tip was removed by fine forceps at 24 ± 1 h APF or 48 ± 1 h APF and placed onto a glass-bottom petri dish (35-mm dish diameter, 14-mm glass diameter, glass thickness 0.16 to 0.19 mm; D11130H, Matsunami, Japan) through a hole made by 1,000-uL pipetman tip to a 250- to 300-μL drop of solidified 1.5% agarose ( ).

Techniques: Sample Prep, Irradiation